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| Contrasting Methods in Life Science |
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| High-Contrast Images of Non-Stained Specimens |
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Target group
Microscopists dealing with transparent biomedical specimens
Prior skills required
Basic skills in light microscopy (e.g., Course on "The Basics of Microscopy")
In many cases, living specimens such as cell cultures, tissue sections or transparent organisms cannot be stained.To image their details in satisfactory contrast, optical contrasting methods are required. Whether phase contrast, DIC, PlasDIC, darkfield or VAREL is the best choice for your specimen depends on many factors. These contrasting methods are increasingly important also for enhancing structures stained with fluorescent dyes and for live cell imaging.
You will learn to select, and perfectly use, the right contrasting method.
- The core of the microscope: choice of the optimum objective
- Brightfield: the classical method
- Darkfield: from limnology to apoptosis
- Phase contrast: the classical method in cellular biology
- DIC (Nomarski differential interference contrast): peerless in developmental biology
- PlasDIC: an innovative method for in-vitro microscopy
- Selecting the best contrasting method for your application
Duration
One day, 9 a.m.- 4 p.m.
Participants
8 | |
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