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| 3D Deconvolution with AxioVision |
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| High Image Definition through Optical Sectioning |
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Target group
Researchers with fluorescence microscopical applications
Prior skills required
Proficiency in fluorescence microscopy and digital imaging (e.g., Courses on "Optimized Fluorescence Microscopy" and "Digital Life Science Microscopy with AxioVision").
Deconvolution is a sophisticated method to improve the
quality of fluorescent image stacks. The 3D Deconvolution module of the AxioVision software from Carl Zeiss is a powerful, yet easy-to-use solution.
This course is intended for users who have not yet worked with 3D deconvolution or wish to optimize their results.
You will learn to set up the hardware and software, to acquire image stacks that are optimum for the method, and to use 3D deconvolution efficiently.
- Methods of optical sectioning (deconvolution, confocal microscopy, structured illumination)
- Basic theory of 3D deconvolution
- Setting up the hardware and software
- Practice of 3D deconvolution
- Inside4D module of AxioVision (creating 3D animations)
Duration
One day, 9 a.m.- 4 p.m.
Participants
6 | |
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