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| Multifluorescence Imaging |
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| and Quantitative Colocalization |
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Prerequisites:
Basic knowledge in Laser Scanning Microscopy is recommended.
Multifluorescence imaging is used to visualize interactions of molecules and other cellular components. The more fluorescent markers are used simultaneously, the more information can be obtained from one experiment. In this context the crosstalk-free separation of fluorescent labels is an essential precondition for reliable results. Colocalization of labeled cellular structures is often used as an indicator for potential interaction. In our course you will learn to use the power of Emission Fingerprinting for crosstalk-free separation of fluorescent labels to evaluate colocalization quantitatively.
- Emission crosstalk, exitation crosstalk and colocalization in Laser Scanning Microscopy: Theoretical principles
- Emission Fingerprinting and Online Fingerprinting
- Application examples: Separation of emission signals from multiple fluorescent proteins, handling of autofluorescent and reflective specimens
- Setup of acquisition parameters for colocalization studies
- Evaluation and quantification of colocalization data
Duration:
two days, 9 a.m. - 6 p.m.
Participants:
6 | |
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