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| Fluorescence Correlation Spectroscopy |
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Prerequisites:
Basic knowledge in light and flourescence microscopy. Basic knowlege in Laser Scanning Microscopy is recommended.
Fluorescence Correlation Spectroscopy (FCS) is a highly sensitive method that detects movement and binding of fluorescence-labeled particles on the single molecule level.
Furthermore, concentrations of movable fluorescent particles can be estimated. Thus, biochemical reactions can be monitored online, in solutions as well as within living cells.
This course will familiarize you with the theoretical background of FCS and train you how to perform and evaluate measurements in solutions and living cells.
- Theoretical fundamentals of Fluorescence Correlation Spectroscopy
- Basic components and functions of the ConfoCor2
- FCS in solutions: Data acquisition, evaluation and optimization
- Image acquisition using the ConfoCor2 - LSM 510
- FCS in living cells: specification of the FCS measurement position within the cell in x,y, and z, data acquisition, evaluation and optimization
Duration:
two days, 9 a.m. – 6 p.m.
Participants:
3 | |
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